Home / Webinars / Gaining Key Phenotypic Data From the Tumor Microenvironment Using 8-plex Immunofluorescence Staining and Image Analysis

Gaining Key Phenotypic Data From the Tumor Microenvironment Using 8-plex Immunofluorescence Staining and Image Analysis
18 December 2020
By Hannah Thomson

Angela Vasaturo, PhD; Senior Scientist, Ultivue
Lorcan Sherry, PhD; Chief Science Officer, OracleBio

Broadcast via The Pathologist, November 2020

Multiplex staining and image analysis are being increasingly deployed for immuno-oncology (I/O) evaluations to investigate and unravel the complexity of immune cell/tumor cell interactions in the tumor microenvironment (TME) and in discovering novel predictive biomarkers for targeted immunotherapy. Immune checkpoint inhibitor treatment, guided by PD-L1 and PD-1 tissue expression assessed by immunohistochemistry, has already shown to improve outcomes for non-small cell lung carcinoma (NSCLC) and colorectal cancer (CRC) metastatic patients. During this webinar, we’ll present a workflow utilizing an 8-plex staining protocol and detailed image analysis approach for the comprehensive assessment of the immune cell types and interactions within the TME, including co-localization and spatial phenotyping analysis in NSCLC and CRC tumor types.

Learning Objectives:

  • A fast, optimized and validated 8-plex multiplex immunofluorescence staining protocol and a comprehensive image analysis can be applied to a broad range of cancer types in order to characterize the immune contexture.
  • The ability to carry out multiparameter cellular analysis facilitates the delivery of key cell phenotypes as well as their functional status, thereby leading to greater understanding of tumor biology.

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