Gaining Key Phenotypic Data From the Tumor Microenvironment Using 8-plex Immunofluorescence Staining and Image Analysis
18 December 2020
By Hannah Thomson

Angela Vasaturo, PhD; Senior Scientist, Ultivue
Lorcan Sherry, PhD; Chief Science Officer, OracleBio

Broadcast via The Pathologist, November 2020

Multiplex staining and image analysis are being increasingly deployed for immuno-oncology (I/O) evaluations to investigate and unravel the complexity of immune cell/tumor cell interactions in the tumor microenvironment (TME) and in discovering novel predictive biomarkers for targeted immunotherapy. Immune checkpoint inhibitor treatment, guided by PD-L1 and PD-1 tissue expression assessed by immunohistochemistry, has already shown to improve outcomes for non-small cell lung carcinoma (NSCLC) and colorectal cancer (CRC) metastatic patients. During this webinar, we’ll present a workflow utilizing an 8-plex staining protocol and detailed image analysis approach for the comprehensive assessment of the immune cell types and interactions within the TME, including co-localization and spatial phenotyping analysis in NSCLC and CRC tumor types.

Learning Objectives:

  • A fast, optimized and validated 8-plex multiplex immunofluorescence staining protocol and a comprehensive image analysis can be applied to a broad range of cancer types in order to characterize the immune contexture.
  • The ability to carry out multiparameter cellular analysis facilitates the delivery of key cell phenotypes as well as their functional status, thereby leading to greater understanding of tumor biology.

More from OracleBio:

Find out more about our Multiplex IF Analysis

OracleBio's Quantitative Digital Pathology Services

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