Angela Vasaturo, PhD; Senior Scientist, Ultivue
Lorcan Sherry, PhD; Chief Science Officer, OracleBio
Broadcast via The Pathologist, November 2020
Multiplex staining and image analysis are being increasingly deployed for immuno-oncology (I/O) evaluations to investigate and unravel the complexity of immune cell/tumor cell interactions in the tumor microenvironment (TME) and in discovering novel predictive biomarkers for targeted immunotherapy. Immune checkpoint inhibitor treatment, guided by PD-L1 and PD-1 tissue expression assessed by immunohistochemistry, has already shown to improve outcomes for non-small cell lung carcinoma (NSCLC) and colorectal cancer (CRC) metastatic patients. During this webinar, we’ll present a workflow utilizing an 8-plex staining protocol and detailed image analysis approach for the comprehensive assessment of the immune cell types and interactions within the TME, including co-localization and spatial phenotyping analysis in NSCLC and CRC tumor types.
- A fast, optimized and validated 8-plex multiplex immunofluorescence staining protocol and a comprehensive image analysis can be applied to a broad range of cancer types in order to characterize the immune contexture.
- The ability to carry out multiparameter cellular analysis facilitates the delivery of key cell phenotypes as well as their functional status, thereby leading to greater understanding of tumor biology.
More from OracleBio:
High ICOS/FOXP3 Tregs Content in the Tumor Microenvironment is Associated with Poorer Survival in Patients with Hepatocellular Carcinoma
OracleBio data quantifying ICOS and FoxP3 dual IHC in clinical HCC samples is presented on a collaborative poster at the AACR 2020 Virtual Annual Meeting. The poster, produced in collaboration with Kymab and the National Taiwan University Hospital, explores the...
Applying deep learning classification for tumor identification across immunohistochemical markers on serial sections to eliminate the need for image co-registration
OracleBio presented a poster at the Society for Immunotherapy of Cancer Annual Meeting 2019 on the 6th – 10th November. The poster is entitled ‘Applying deep learning classification for tumor identification across immunohistochemical markers on serial sections to...
Characterization of Anti-CTLA-4 Effects on Tumor Growth and Immune Microenvironment in a Syngeneic CT26.WT Colon Carcinoma Mouse Model
In collaboration with Pharmatest, OracleBio presented a poster at the AACR Annual Meeting 2019, which took place from the 29th of March to the 3rd of April 2019. The poster is entitled ‘Characterization of Anti-CTLA-4 Effects on Tumor Growth and Immune...
Find out more about our Multiplex IF Analysis
OracleBio's Quantitative Digital Pathology Services
Stay up to date with OracleBio
Sign up to our mailing list to stay informed about news, webinars, events and more