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FAQs

Getting Started

What if I don’t know exactly what I want to measure?

We can provide advice on the best approach for your image analysis based on our experience in the area. Contact us to arrange an initial study consultation and we will be happy to help.

How much does it cost?

The cost of the image analysis is dependent on a number of factors including, size and complexity of the tissue, the number of parameters to be quantified and the amount of manual corrections required to remove artifacts etc. Please contact us and we would be happy to discuss your requirements and provide you with a quote for the work.

How long will it take to receive my data?

Turnaround time will depend on the total number of slides and markers to be analysed.  Please contact us and we would be happy to discuss your requirements and provide you with timelines for your study.

What if I only have tissue slides and no images?

You can send your histology slides to us and we can convert them to digital images using our Hamamatsu Nanozoomer slide scanner. We can then carry out the analysis on the digital images and send the data and images back to you.

Practical Questions

What’s the best way to transfer images to you?

OracleBio have a secure server which allows easy upload and download of images and data from your desktop computer. Contact us and we can provide you with login details to your own folder.

What type of image formats do you accept?

Our software works with most image formats (tiff, jpeg etc). We can work with whole slide images generated from most commercially available scanners including Aperio, Leica, Olympus, Hamamatsu, Mirax, 3D Histech etc.

Would you be prepared to work with our current preferred CRO’s / Suppliers?

Yes, we work with a number of partner companies and would be happy to work alongside your preferred suppliers.

Technical Questions

Can you analyse formalin fixed paraffin embedded tissue?

Yes. We can perform analysis on tissue that has been fixed using any established method. Good tissue quality is paramount and will affect both the Histology/IHC performed and subsequent image analysis.

Can you analyse frozen tissue sections?

Yes. We can perform analysis on frozen tissue sections. Remembering that good tissue quality and slide storage is paramount and will affect both the Histology/IHC performed and subsequent image analysis.

Can you measure data across the whole section?

Yes, we use software which allows analysis across the whole tissue section or particular regions of interest within the tissue section, depending on your requirements.

I need a histological score measurement, do you do this?

Yes, we can provide histological score measurements on a scale of 0 – 300, representative of all the cells analysed in the whole tissue section or region of interest.

Do you analyse multiplex staining?

Yes, we provide quantification of both multiplex chromogenic and fluorescence images.

Can you analyse IMC images?

Yes, we can analyse IMC images to provide object data per cell. We can also provide bioinformatical analysis of this data via our links with partner companies.

Additional Services

Do you provide Pathology review services?

Yes. We have internal pathologist capabilities to provide Pathology review services to support your study. We also work with an extended network of pathologists across the EU and US. Visit the Pathology section of our website to find out more.

Do OracleBio also provide Histology and IHC services?

We can Histology / IHC services via our links with partner companies. For more details see the Study Management Services section of our website.

Can you also procure tissue?

Yes, we can procure specific tissues for your studies via our network of partner companies. See the Study Management Services section of our website for more details

Reports

What would the final data package include?

The data package will include image analysis raw data files (csv), Excel data summary tables, image analysis screenshots (jpeg), original images and, if required, a detailed report.

What format are your reports?

We provide either a PowerPoint or a Word report, depending on the client’s preference.

Can OracleBio supply a report with statistics?

Yes, we perform statistical analysis for many of our clients and all data will be included in the final report document. We are happy to perform analysis in a blinded fashion with specific statistical analysis being carried out once the data have been generated.

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OracleBio's Quantitative Digital Pathology Services

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