providing high precision, quantitative, digital image analysis services to support pre-clinical and clinical research

Frequently Asked Questions

We can provide advice on the best approach for your image analysis based on our experience in the area. We routinely carry out image analysis algorithm optimisation work on pilot samples from our clients and then arrange an online meeting to discuss the details to allow changes to be made before proceeding with the analysis of the main study.

You can send your histology slides to us and we can convert them to digital images using our Hamamatsu Nanozoomer slide scanner. We can then carry out the analysis on the digital images and send the data and image back to you.

Yes. We can perform analysis on tissue that has been fixed using any established method. Good tissue quality is paramount and will effect both the Histology/IHC performed and subsequent image analysis.

Yes, we use software which allows analysis across the whole tissue section or particular regions of interest within the tissue section, depending on the clients preference.

We can provide detailed analysis across any whole slide image or specific/specified region of interest within that tissue. This can be quantification for an area of marker staining or at a cellular level to deliver accurate cell-by-cell quantification of membrane, cytoplasm or nuclear staining.

We can perform detailed quantitative analysis on a cell-by-cell basis across the whole slide image. Cells immunohistochemically stained with either a membrane, cytoplasmic or nuclear marker can be positively identified and quantified as individual cells or as an area measurement across the whole tissue section.

Yes, we can quantify immunofluorescent labeled antibodies, either as single label IHC study or as part of an IHC multiplex study (allowing for colocalisation to 2 specific markers in the same tissue / cell). We can further use specific fluorescent labels to identify particular regions of interest in a tissue (for example Pan CK for tumor tissue) and then determine a target specifc label within the region of interest. For some examples of our fluorescence work please see our image analysis gallery.

Yes we can perform colocalisaton of  markers in the same tissue. For colorimetric stained sections we can quantify the area of two markers (red and brown) plus the area of colocalised read and brown on the same section. For fluorescent stained sections, we can quantify the number of cells which are positive for either specific marker and the number of cells that are positive for both colocaliased markers.

No Images, no problem! We can scan your slides using our Hamamatsu Nanozoomer and return the whole slide digital images to you via our secure cloud server and/or on a portable hard drive.

Yes, our software enables us to quantify parameters within TMA cores from both colorimetric and fluorescent stained slides. Each core is deteced as an individual tissue and marker staining quantified as an area or on a call by cell basis to give a postive cell count or Histological score (IHC intensity). We can also perform cell compartment analysis on TMA cores to quantify marker staining speicifically within the nucleus, cytoplasm or membrane of each cell. See the TMA example in Image Analysis Gallery for more details.

Yes. We can perform analysis on frozen tissue sections. Remembering that good tissue quality and slide storage is paramount and will effect both the Histology/IHC performed and subsequent image analysis.

Yes, OracleBio can supply quality non-GLP and GLP Histology / IHC services via our links with partner companies. For more details see the partner services section of our website.

No problem. We offer a slide scanning service using our Hamamatsu Nanozoomer slider scanner. Slides can be scanned at x20 or x40 magnification in bright field or fluorescence mode. For more details, see the Slide Scanning section of our website.

Yes, we perform statistical analysis for many of our clients and all data will be included in the final report document. We are happy to perform analysis in a blinded fashion with specific statistical analysis being carried out once the data have been generated.

The cost of the image analysis is dependent on a number of factors including, size and complexity of the tissue, the number of parameters to be quantified and the amount of manual corrections required to remove artifacts etc. Please contact us and we would be happy to discuss your requirements and provide you with a quote for the work.

Turnaround time will depend on the total number of slides and markers to be analysed.  For example, for 50 slides analysing a single marker on a whole tissue section, data would be returned to the client within 1-2 weeks of receiving the digital images.  Data would be returned to the client as soon as the analysis and quality control was complete, release of a formal report of the data would take longer.

Our software works with most image formats (tiff, jpeg etc). Higher quality images, such as those produced by a digital scanner, will provide better quality data and we can work with whole slide images generated from a wide range of scanners including Aperio, Leica, Olympus, Hamamatsu, Mirax, 3D Histech etc.

Yes, we can provide histological score measurements in an objective and reproducible manner using our software. Each cell within a tissue can be individually detected and classified based on their marker IHC intensity as negative, positive low, positive medium or positive high. A histological sore is then generated on a scale of 0 – 300, representative of all the cells analysed in the whole tissue section or region of interest. Statistical analysis can be performed on tissue group means for interpretation of response.

Yes, we have strong links with clinical and CAP registered veterinary pathologists who can provide a review of your slides, selection/guidance of specific regions of interest for analysis and generateion data reports.

The data package will include image analysis raw data files (csv), Excel data summary tables, image analysis screenshots (jpeg), original images and, if required, a detailed report.

Yes, we have worked on a number of clinical trials and are happy to work with you and/or your preferred CRO of interest to deliver your image analysis requirements.

Yes, we have a number of years of experience in optimising IHC protocols, gained whilst working in the target validation departments of large pharma. We can advise on best practice for optimal storage, processing and staining of tissues to provide quality image analysis data.

Yes, OracleBio have a secure server which allows easy upload and download of images and data from your desktop computer. Contact us and we can provide you with login details to your own folder.

Yes, we can procure specific tissues for your studies via our network of partner companies. See the Partner Services section of our website for more details

We are happy to coordinate your full study needs including working with various partner organisations to deliver the solution that you require. We have project management qualifications and have many years of experience of project managing studies.

Yes, we work with a number of partner companies and would be happy to work alongside your preferred suppliers.

FAQ didn’t solve your problem?

Contact us to allow us to answer your questions and discuss your requirements