Renal (kidney) fibrosis is the principal process underlying the progression of chronic kidney disease (CKD) that can lead to organ failure and eventual death. Using Masson’s trichrome or Picrosirius red IHC staining techniques together with image analysis approaches, it is possible to quantify the collagen content within fibrotic kidney tissue. Quantifying collagen levels together with cellular content in models of kidney fibrosis, such as the unilateral ureteral obstruction (UUO) model, can be used to determine the effect of drug treatment on kidney fibrosis.
Fibrotic tissue section showing collagen (green) detection in cortex
Histological stains such as Picrosirius Red or Masson’s TriChrome can be used to reveal inflammatory and fibrotic changes within kidney tissue sections. A custom algorithm is developed to accurately identify and segment cortex areas within the whole tissue section. Levels of collagen deposition within the kidney cortex region of interest (ROI) can be accurately quantified and classified.
Magnified fibrotic kidney section showing collagen (green) being detected in the cortex
Total area of kidney tissue section
Area of cortex
Area of collagen deposition within the cortex region of interest (ROI)
% collagen relative to area of cortex ROI
Assessment over whole slides rather than individual field of views
Mouse Kidney Fibrosis Data
Custom algorithms allow accurate classification of cortex region of interest (ROI)
The ability to quantify and classify the area of collagen staining within the cortex
Objectively applied analysis across all study slides
Whole slide quantitative analysis increases the robustness of the data
Whole slide image overlays of ROI and collagen detection returned to the Client