Microvessel density (MVD) IHC is often used as a surrogate measure of angiogenesis in tumour tissue with angiogenesis being correlated with poor disease prognosis and/or therapeutic response.
CD34 positive cells
Using immunohistochemistry (IHC) techniques with specific antibodies directed against endothelial cells (eg CD34 ) it is possible to stain and identify endothelial cells within microvessels and using image analysis, quantify the number and size of microvessels within a tissue.
Detected vessels (purple) and lumen (green).
Total number of vessels within a tissue section
Total area of vessels within a tissue section
Number of small, medium and large vessels
Number of vessels with or without lumen
Objective analysis across whole tissue slides rather than subjective individual field of views
Vessels classified small (yellow), medium (orange) and large (red).
Custom angiogenesis algorithms allow highly accurate detection of vessel formation
A highly detailed measure of new vessel formation across the whole tissue section or specific region of interest
The ability to not only quantify but also classify vessels into various sizes
Whole slide analysis increases the robustness of the the data