Figure 1. (A) Magnified tissue section with IHC staining showing CD3 single positive cells (purple), LAG-3 single positive cells (brown), LAG-3/CD3 dual positive (purple/brown) and nuclear counterstain (blue). (B) Deconvoluted image showing CD3 positive staining. (C) Deconvoluted image showing LAG3 positive staining. (D) Image analysis overlay, where cytoplasmic borders for CD3 positive cell (light purple), LAG-3 positive (brown), LAG-3+/CD3+ dual positive (purple/brown) and nuclei (blue).