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NAS Score and Pathologist Review
NAS score for Pre-clinical NASH models
Non-Alcoholic Steatohepatitis (NASH) is a chronic liver disease clinically characterized by hepatic steatosis, lobular inflammation and fibrosis.
In pre-clinical research, various animal models have been shown to mimic the histopathological features and stages of NASH.
The NASH/NAFLD Activity Score (NAS) is a scoring system that semi-quantitatively evaluates and grades NASH/NAFLD based on the following core histopathological features: steatosis (0-3), lobular inflammation (0-3), hepatocellular ballooning (0-2), and fibrosis (0-4).
The use of NAS scoring has been shown to be a reproducible and reliable scoring system suited for the assessment pre-clinical murine models of NASH and Liver Fibrosis.
In collaboration with one of our CRO partners, OracleBio can provide full histology and IHC services and a range of NASH/NAFLD and Fibrosis qualitative assessment read-outs to complement our quantitative image analysis data.
Figure 1. Example Hematoxylin and Eosin (H&E) Stained NASH Model Tissue
NAS Score Read-outs:
We can provide individual disease progression read-outs and a combined NAS Score per sample:
Lobular Inflammation (0-3)
Hepatocyte Injury (0-2)
Quantitative Image Analysis Read-outs:
We can provide quantitative image analysis read-outs based on:
Steatosis – Vacuole Count/Area/Size/Diameter
Fibrosis – Collagen Content/Area
Lobular Inflammation – Cell Count based on Immune/Inflammatory IHC specific staining