Get in touch +44 (0)141 255 1640 | email@example.com
Dual Immunohistochemistry (IHC)
Dual Immunohistochemistry (IHC) analysis can be applied to a variety of IHC applications. It allows for the identification of two different cell populations or co-localisation of two antigens to one cell on a single tissue section.
Analysis Approach We develop a customised algorithm tailored to the specific markers in your study. This involves a 3-chromogen color deconvolution process within the Indica Labs HALO platform to separate the 2 IHC chromogens plus counterstain. Cell objects are formed by applying weighted optical density values for the individual chromogens. Each positive cell type is then identified using defined size, shape and subcellular compartment staining parameters. A classifier is developed and integrated into the algorithm to automatically segment the tissue region of interest (ROI) for analysis. The completed algorithm is applied to all tissue section images in the study in an automated and objective manner to generate detailed cell-by-cell data. Example analysis and data is shown below. For further analysis of dual IHC analysis please click here.
A. IHC stained whole tissue section (left) and classifier overlay (tumor – orange; stroma – blue; white space – white) B. IHC stained tissue section (left) and cell detection of beta-Catenin (brown) and Cyclin D1 (teal) within the viable tumor ROI. Histology performed by Propath UK.
Area of specific tissue regions of interest (ROI) including viable tumor and stroma content across the whole section
Quantification of cells positive for multiple chromogenic markers across a whole tissue section or within a defined ROI
Quantification of cells in which multiple chromogenic markers co-localise
Example Dual IHC Analysis Data
Receive highly detailed data quantifying multiple single markers or colocalised markers on a cell-by-cell basis across a whole tissue section or within a defined ROI.
Our automated batch analysis service objectively analyses each section using the same algorithm to reduce variability and improve data quality and interpretation.
Click on the different category headings to find out more. You can also change some of your preferences. Note that blocking some types of cookies may impact your experience on our websites and the services we are able to offer.
Essential Website Cookies
These cookies are strictly necessary to provide you with services available through our website and to use some of its features.
We provide you with a list of stored cookies on your computer in our domain so you can check what we stored. Due to security reasons we are not able to show or modify cookies from other domains. You can check these in your browser security settings.
Google Analytics Cookies
These cookies collect information that is used either in aggregate form to help us understand how our website is being used or how effective our marketing campaigns are, or to help us customize our website and application for you in order to enhance your experience.
If you do not want that we track your visit to our site you can disable tracking in your browser here:
Other external services
We also use different external services like Google Webfonts, Google Maps, and external Video providers. Since these providers may collect personal data like your IP address we allow you to block them here. Please be aware that this might heavily reduce the functionality and appearance of our site. Changes will take effect once you reload the page.
Google Webfont Settings:
Google Map Settings:
Google reCaptcha Settings:
Vimeo and Youtube video embeds:
The following cookies are also needed - You can choose if you want to allow them: