Renal (Kidney) Fibrosis

Renal (kidney) fibrosis is the principal process underlying the progression of chronic kidney disease (CKD) that can lead to organ failure and eventual death. Using Masson’s trichrome or Picrosirius red IHC staining techniques together with image analysis approaches, it is possible to quantify the collagen content within fibrotic kidney tissue. Quantifying collagen levels together with cellular content in models of kidney fibrosis, such as the unilateral ureteral obstruction (UUO) model, can be used to determine the effect of drug treatment on kidney fibrosis.

Fibrotic tissue section showing collagen (green) detection in cortex

Fibrotic tissue section showing collagen (green) detection in cortex

Analysis Approach

Histological stains such as Picrosirius Red or Masson’s TriChrome can be used to reveal inflammatory and fibrotic changes within kidney tissue sections. A custom algorithm is developed to accurately identify and segment cortex areas within the whole tissue section. Levels of collagen deposition within the kidney cortex region of interest (ROI) can be accurately quantified and classified.

Magnified fibrotic kidney section showing collagen (green) being detected in the cortex

Magnified fibrotic kidney section showing collagen (green) being detected in the cortex

Quantitative Readouts

  • Total area of kidney tissue section
  • Area of cortex
  • Area of collagen deposition within the cortex region of interest (ROI)
  • % collagen relative to area of cortex ROI
  • Assessment over whole slides rather than individual field of views

Mouse Kidney Fibrosis Data

Benefits

  • Custom algorithms allow accurate classification of cortex region of interest (ROI)
  • The ability to quantify and classify the area of collagen staining within the cortex
  • Objectively applied analysis across all study slides
  • Whole slide quantitative analysis increases the robustness of the data
  • Whole slide image overlays of ROI and collagen detection returned to the Client