Cell Proliferation IHC Quantification

Quantification of cellular proliferation using nuclear proliferation markers such as Ki67, BrdU and PCNA via immunohistochemistry (IHC) techniques can be used to determine the activity of drugs that modify cell turnover.

Cellular Proliferation Overlay

Cellular Proliferation Overlay

Analysis Approach

Cell proliferation quantification involves detecting the number of positive and negative stained nuclei. Whole tissue sections are digitally scanned and then detailed, high magnification cell by cell analysis is used to detect presence of the chosen proliferation marker eg Ki67, BrdU and PCNA.  Quantification of cell proliferation can be objectively applied over the entire tissue or a specified area of tissue.

Quantitative Readouts

Cell proliferation quantification parameters can include:

  • total number of nuclei across whole tissue sections or specific morphological regions of interest
  • number of positive and negative stained nuclei
  • quantification of both proliferating  and non-proliferating cells
  • quantification of the Proliferation Index
Cellular Proliferation whole tissue data

Cellular Proliferation whole tissue data

Benefits

We provide highly accurate and quantitative cell proliferation data with the following benefits:

  • Data can be expressed as a Proliferation Index rather than % area stained which improves data quality
  • Cell proliferation can be quantified in specific tissue regions of interest, for example viable but not necrotic tissue in Xenograft sections
  • Customised algorithms are developed for each specific study including defining precise IHC thresholds for detection of true positive nuclear staining and exclusion of artefacts (i.e. histological deposits) to avoid false positive data points
  • Cell proliferation can measured using Ki67, BrdU and PCNA approaches
  • Whole slide analysis increases the robustness of the data with total nuclei counts exceeding 100,000 depending on tissue size