Angiogenesis IHC Analysis
Microvessel density (MVD) IHC is often used as a surrogate measure of angiogenesis in tumour tissue with angiogenesis being correlated with poor disease prognosis and/or therapeutic response.
CD34 positive cells
Using immunohistochemistry (IHC) techniques with specific antibodies directed against endothelial cells (eg CD34 ) it is possible to stain and identify endothelial cells within microvessels and using image analysis, quantify the number and size of microvessels within a tissue.
Detected vessels (purple) and lumen (green).
- Total number of vessels within a tissue section
- Total area of vessels within a tissue section
- Number of small, medium and large vessels
- Number of vessels with or without lumen
- Objective analysis across whole tissue slides rather than subjective individual field of views
Vessels classified small (yellow), medium (orange) and large (red).
- Custom angiogenesis algorithms allow highly accurate detection of vessel formation
- A highly detailed measure of new vessel formation across the whole tissue section or specific region of interest
- The ability to not only quantify but also classify vessels into various sizes
- Whole slide analysis increases the robustness of the the data