Utilizing multiplex chromogenic IHC and digital image analysis to evaluate immune cell content and spatial distribution within NSCLC tumor tissue

OracleBio presented a poster at the 2017 Annual Meeting of the American Association for Cancer Research (AACR), which took place from the 1st to the 5th April in Washington D.C. The poster, made in collaboration with Asterand Bioscience, is entitled ‘Utilizing multiplex chromogenic IHC and digital image analysis to evaluate immune cell content and spatial distribution within NSCLC tumor tissue’ and is provided in full below.

Click to view or download the poster

AACR poster: Utilizing multiplex chromogenic IHC and digital image analysis to evaluate immune cell content and spatial distribution within NSCLC tumor tissue

The poster shows data from a study in which a customized algorithm was applied to triplex chromogen IHC plus hematoxylin stained Non-Small Cell Lung Carcinoma (NSCLC) tumor tissue in order to quantify CD8 and FoxP3 immune cell content and spatial distribution within both tumor and stroma regions of interest (ROI).

The study concludes that the use of multiplex IHC combined with digital image analysis is a valuable approach for quantifying the content and spatial distribution of multiple cell types within the context of their tumor microenvironment and in relation to target positive tumor cells. Furthermore, up to five chromogen colors can be separated utilizing a custom 5-plex analysis algorithm, leading to a more in-depth evaluation of immune cell types present and their spatial distribution. This propounds a greater potential for interpretation of therapeutic mechanistic responses in relation to the immune contexture within the tumor microenvironment.

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